Kazhal Ghanbari - Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, Iran
Mahmoud Roushani - Department of Chemistry, Faculty of Sciences, Ilam University, Ilam, Iran
Azadeh Azadbakht - Department of Chemistry, Khorramabad Branch Islamic Azad University Khorramabad, Iran

In this report, a new aptamer-based assay was presented reporting the electrochemical aptasensing for sensing tyrosinamide (Tyr-NH۲). This strategy was relied on unbeatable conformational flexibility and specific recognition of aptamers. The tyrosinamide aptamer (Tyr-NH۲-aptamer) was immobilized onto the metal-organic frameworks/silver nanoparticles modified glassy carbon electrode and hexacyanoferrate was selected as a probe to monitor interface variations during modification of the electrode and the aptamer conformational change generated by the Tyr-NH۲ binding. Results showed that measurements by using electrochemical impedance spectroscopy had linear with the Tyr-NH۲ concentrations in range of ۰.۰۱-۰.۲۵ nM and ۰.۲۵-۱.۱۵ nM. Detection limit of this system was found to be ۲.۳ pM. This method was also used to the Tyr-NH۲ detection in serum samples successfully. Remarkable simplicity, ease of use and low-cost, make methodology as sensitive analytical system for sensing of the Tyr-NH۲ that can be miniaturized. This strategy offers some promising advantages in reliable detection of the Tyr-NH۲, which may be helpful in the routine analysis.

Electrochemical aptasensor, Tyrosinaimde, Hexacyanoferrate, Metal-organic Frameworks, Silver nanoparticls