Effects of gamma irradiated Avian Influenza vaccine subtype H9N2 on vaccinated chicken’s splenic cell proliftration

IntroductionInfluenza is one of the most important diseases in the poultry industry. It has adverse economic effects on the poultry industry as well as public health, causing numerous epidemic effects in different countries,and our country is no exception. In the middle of July 1998, this virus caused a kind of unknown illness in poultry around Tehran and Qazvin. It was then isolated and identified under the Type A/chicken / Iran / 259/1998 H9N2 in Razi vaccine and serum research institute of Iran and Tehran Veterinary School. Unfortunately, the disease has spread rapidly in the country, and despite the special plans, the disease has been endemic and has caused extensive damage to the country s poultry industry.The aim of this research is production of gamma irradiated Avian Influenza subtype H9N2 antigen and considering its effects on proliferation of chicken’s spleeny T lymphocyte.Methods and Materials:Avian Influenza virus (AIV) subtype H9N2 was multiplied on embryonated SPF chicken eggs of 9–11 days. AIV was irradiated by a Nordian model 220 gamma cell instrument at a dose rate of 2.07 Gy/s and activity of 8677 Ci to damage influenza virus genomic RNA to inactivate virus infectivity with 30 kGy gamma radiation. The spleeny T lymphocyte proliferation was evaluated on vaccinated broiler chickens by three vaccine concentrations (1, 10-1, and 10-2) and injection method according to the MTT test. The result of stimulation index was analysis by Anova one-way method.ConclusionThe result of MTT test was shown proliferation of spleeny T lymphocyte was increase significantly for two vaccinated groups (1 and 10 -1 vaccine concentrations) against negative control group (P<0.05).

کلیدواژه ها:

Avian Influenza Virus ، Gamma Irradiation ، Inactivation ، Splenic Lymphocyte ، MTT test