Oseltamivir efficacy against avian influenza A virus (H9N2) replication in cell culture in kazeroun city

Objectives: The low pathogenic avian influenza H9N2 subtype virus is one of the member of the influenza family which has been widely circulating in different birds, mammalian species and even human population. Although vaccination is the most efficient method to control the disease but some difficulty were included this approach. Currently two classes of antiviral drugs have been approved: M2 ion channel and neuraminidase inhibitors. In this experimental assay the anti-avian influenza virus (H9N2) activity of Oseltamivir was evaluated.Materials & Methods: MDCK cells culture were grown in 96 well plates for 48 hours and inoculated with influenza virus (H9N2) at MOI=0.1 for one hour, then oseltamivir were added at different concentration(0.5-0.005 mg/ml), viral titration were carried out by TCID50, HA and Real Time PCR tests, also Cell viability of oseltamivir investigated by MTT test.Results & Conclusion: the Cytotoxicity concentration 50% of oseltamivir was 0.5mg/ml. Oseltamivir exhibited a dose dependent anti avian influenza virus (A/chicken /Iran/772/1998(H9N2) activity. Virus titers were reduced at 0.5-0.005 mg/ml in all of experimental tests and interestingly a drastic decrease in the viral genome copy number were observed in all of concentrations.The findings of our study, suggest that oseltamivir has a potent anti avian influenza activity and it could be a choice for control and treatment of avian influenza subtype H9N2 in poultry industries.

کلیدواژه ها:

avian influenza ، oseltamivir ، antiviral ، cell culture ، Real Time PCR