Attenuation of inflammation genes expression by Juglans regia extract in an Osteoarthritis in vitro like-model.

Today, the use of herbal medicine in common disease control and treatment, such as inflammatory diseases; is increasing. The present study aimed to assess anti-inflammatory effects of Juglans regia extract in an osteoarthritis-like model on synoviocytes cells cultured from Holstein cows. the extract cytotoxicity was determined as 1mg/dL by MTT, LDH and SDH assay. Then LC50 was determined as 1µg/ml. The pre-inflammatory cytokines TNF-α, IL-18 and IL-1β genes expression treated with NSAIDs, Steroids and the extract was assessed in groups as followings; Cell, Cell+LPS, Cell+LPS+NSAIDs, Cell+LPS+Steroids and Cell+LPS+Juglans regia extract. Then using real-time PCR technique, analyzed with Pffafl, F static and ANOVA methods and contrasting with REST 2009 software. The results showed down-regulating expression of the inflammatory genes (p<.05), due to Juglans regia extract and anti-inflammatory effects of the extraction. In the present study, it proved that effects of Persian walnut extract on synoviocytes decreased the expression of genes involved in inflammation 42.37%,47.53% and 42.81% respectively for TNF-α, IL-1β and IL-18; contrasting to control group. So Persian walnut ingredients can be used as an alternative treatment and it can be offered as well as an effective recommended.

کلیدواژه ها:

anti-inflammatory effects ، Juglans regia extract ، cytokines ، synoviocytes ، gene expression ، qPCR.