Structural and functional studies of plant Polyamine Oxidase

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 361

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شناسه ملی سند علمی:

CIGS15_027

تاریخ نمایه سازی: 13 بهمن 1398

چکیده مقاله:

Intracellular polyamine contents are regulated by not only biosynthesis and transport but also catabolism by FAD dependent polyamine oxidases (PAOs). Results of various studies on PAO proteins in developmental processes and response to environmental stresses confirm the importance of this protein in plant life, however, there is no comprehensive study of phylogenetic and structural relationships of plant PAOs. In the present study, in order to better understand phylogenetic and structural relationships of PAO proteins, bioinformatics analysis of 58 PAO protein sequences of 15 different plant species were performed. Multiple clusters with gene duplications were identified in both dicot as well as monocot-species. According to the conserved motifs obtained by MEME and MAST tools, four motifs were common in most plant species. Structural analysis was carried out on PAOs from Oryza sativa and Arabidopsis thaliana as representative of monocot and dicot plants, respectively, as no structural information is available on them. Secondary structure analysis revealed that alpha helix dominated among secondary structure elements followed by random coils, extended strand and beta turns for all sequences. Tertiary structures were predicted with SWISS-MODEL server. The structural, physicochemical, and phylogenetic information predicted in this study will provide a good foundation for functional analysis of the plant PAOs proteins.

نویسندگان

Mohsen Hosseini

PhD Student, Professor, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C, Tehran, IRAN

Abbas Saidi

PhD Student, Professor, Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C, Tehran, IRAN