Application of allele specific markers in determining alleles at VRN-1 loci in bread wheat genotypes

Wheat is the most important crop and is cultivated all over the world. Wheat has a wide range of adaptability to different climates. Vernalization genes are one of the important factors determining wheat adaptability. Knowing the presence of vernalization gene is important to introduce new cultivars for different regions. At a molecular level, the length of the vernalization period of common wheat (Triticum aestivum L.) is determined mainly by three loci: VRN-1, VRN-2 and VRN-3. The VRNA1, VRN-B1, and VRN-D1 genes are dominant for spring growth habit and epistatic to the alleles for winter growth habit. Therefore, winter cultivars are homozygous for the recessive alleles at the three VRN-1 loci. In the current study, 34 bread wheat accessions plus Chinese Spring wheat were investigated for allelic variation at Vrn1 loci. The current study showed that at VRN-A1 five genotypes had dominant allele. VRN-A1b allele was not found at any of the genotypes. Only one genotype had VRN-A1c allele and 32 genotypes including the Chinese Spring showed vrn-A1 allele. The genotype No. 6 unexpectedly had more than one allele. At locus VRN-B, 14 genotypes had dominant allele while 20 including the Chinese Spring showed recessive allele.

کلیدواژه ها:

Allelic variation ، Bread wheat ، Molecular markers ، Vernalization