Optimization of some effective in vitro factors in micropropagation of Stevia rebaudiana Bertoni

Stevia, as an anti-diabetic medicinal plant, belonging to family Asteracae, The leaves of Stevia are source of diterpene glycosides, suchas stevioside and rebaudiosides which are estimated to be about 300 times sweeter than sugar cane. Seeds of stevia is very small and they have a very low germination percentage, Propagation by seeds doesnot resulting homogenous plant population leading to a great variability in the important features like sweetening levels and compositions. therefore, micropropagation by tissue culture technology is an appropriate method for rapid, massive and uniform proliferation in stevia. In the present research, the effect of various basal medium culture and different hormones on in vitro micropropagation of stevia were investigated. Single node explants were cultured in MS and B5 medium culture containing BAP and NAA. The results showed that the kind of media culture and the rate and concentration of plant growth regulators had significant effect on growth characters and regeneration of stevia. The maximum leaf and node number and shoot length were obtained in Ms medium culture containing 0.5 mg l-1 NAA and 1 mg l-1 BAP. The obtained results revealed that, the use of suitable culture medium with growth regulators for the micropropagation of stevia increases plant growth

کلیدواژه ها:

Stevia ، BAP ، NAA ، medium culture ، B5 ، Ms.