Investigating the effect of Ibuprofen on the expression of Notch1 transcript in gastric cancer stem cells derived from MKN-45 cell line

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 362

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شناسه ملی سند علمی:

CIGS15_179

تاریخ نمایه سازی: 13 بهمن 1398

چکیده مقاله:

Gastric cancer is the third cause of death worldwide. In spite of progressing in the treatment of cancers, it can recur tumour and lead to metastasis. While on the other hand, diagnosis of this disease is poor. Therefore, a new therapeutic approach is needed to improve the clinical outcome of gastric cancer therapy. Cancer stem cells are identified in a lot of solid malignancies and we can target these cells to eradicate cancer [1]. Although they form only a small population of cancer, but cancer stem cells (CSCs) play a pivotal role in cancer progress [2]. We have investigated if targeting CSC by nonsteroidal anti-inflammatory drugs (NSAIDs) can be useful for treatment of gastric cancer. NSAIDs can relief pain and control inflammation. Also NSAIDs inhibit cyclooxygenase (COX) activity and the synthesis of prostaglandins. Many studies have suggested that ibuprofen, which is a type of NSAIDs, inhibits the progress and proliferation of some cancers. These drugs affect the regulation of various signalling pathways (for instance Notch pathway), that has been disrupted during tumour formation [3]. So far, no study has been done to determine the effects of ibuprofen on gastric cancer stem cells derived from MKN-45 cell line. To this end, we had studied that what is the impacts of ibuprofen on the expression of NOTCH1. To this end, we used qRT-PCR technique. Our findings provide that ibuprofen can reduce the proliferation of gastric cancer stem cell by down-regulating this gene. The results demonstrated that in gastric cancer stem cell, NOTCH1 has an oncogenic role. Above finding suggest inhibition of Notch signalling resulted in decreased NOTCH1 expression in response to ibuprofen.

نویسندگان

F Azarafrouz

Department of Biology, School of Sciences, Razi University, Kermanshah, ۶۷۱۴۹-۶۷۳۴۶, Iran

H Fallahi

Department of Biology, School of Sciences, Razi University, Kermanshah, ۶۷۱۴۹-۶۷۳۴۶, Iran

H Akrami

Department of Biology, School of Sciences, Razi University, Kermanshah, ۶۷۱۴۹-۶۷۳۴۶, Iran