Discovery of Gene Networks contribute in Chronic Lymphoid Leukemia (CLL) through Enrichment Analysis

Introduction: According to reduction in time and cost of Massively Parallel Sequencing (MPS), genomic information from malignancies becomes more available. CLL is a hematological malignancy in which genetic alterations in different genes have been suggested using Next Generation Sequencing (NGS) in recent years.Aim: In this study we have determined involved gene networks in this disorder according to the gene ontologies and molecular pathways.Methods: We reviewed all 36 articles in which whole exome sequencing have been used in CLL patients. We have found 41 important affected genes in this disorder. In order to determine the gene networks that were involved, network based enrichment analysis for gene ontologies and molecular pathways was done using EnrichNet Online tool[1]. Pathways or processes by 3 or more affected genes and significant fisher exact test (p<0.05) are presented.Results: Using GO molecular function, contribution of 14 affected genes in DNA binding process was significant. In GO cellular Component, 22 genes with nucleus products, 11 genes which compose nucleoplasm and 4 genes with products in telomeric region showed significant contribution. Cellular response to UV, B cell activation and B cell receptor signaling pathway were involved in GO biological process. In Corum (MIPS) complexes, DDX3X and SF3B1 in spliceosome were affected. Apoptosis and cell cycle genes were involved in KEGG pathways. Apoptosis, miRNAs involved in DDR and TP53 network were involved in wiki pathways. ACD, POT1 and TERF1 in packaging of telomere ends were found in Reactome. In NCI pathway interaction DB also telomerase pathway/Regulation of Telomerase was involved.Conclusion: Involvement of genes in apoptosis (TP53, BIRC3) and telomere structure/function (ACD, POT1, TERF1) were common consensuses in network based enrichment analysis of CLL

کلیدواژه ها:

Enrichment Analysis ، Chronic Lymphoid Leukemia