Molecular diagnosis of Nodularin/Microcystin producing Cyaobacteria in Persian Gulf

Introduction: Cyanobacteria are important producers in nature. Of the well-known cyanobacteria, about 40 species are the main cause of blooming of the poison, which causes damage to organisms in nature and in humans. Determining the presence of cyanobacteria and their dangerous toxins in water is a major issue in the environment and drinking water. There are several methods for investigating the presence of cyanobacteria producing Nodularin/Microcystin, but each has its own limitations. In this study, the presence of Nodularin/Microcystin on the coast of the Persian Gulf has been investigated by the PCR method. Materials and Methods: In this research, sampling was done from 20 stations along the Persian Gulf coast and DNA extraction from water samples was done using modified DNG-Plus method. PCR test for the presence of cyanobacteria with universal primers (CYA359F, CYA781R) and the detection of cyanobacteira producing Nodularin/Microcystin by assigning primer (HEPF, HEPR) and optimal sensitivity and specificity were studied. Then PCR experiments were performed on samples of the Persian Gulf.Results: Positive optimized universal PCR test on samples indicates the presence of cyanobacteria in the Persian Gulf was studied in 20 stations. Of which cyanobacteria’s producing Nodularin/Microcystin was confirmed in 7 stations.Conclusion: Due to the presence of Cyanobacteria in all stations sampled, we can say, these organisms, predominant in the Persian Gulf and the Cyanobacteria producing Nodularin/Microcystin in 35% of sampling stations showed the dangers in the area of the cyanobacteria producing the toxins in the Persian Gulf.

کلیدواژه ها:

Nodularin/Microcystin ، PCR ، Persian Gulf ، Diagnosis