Investigating of CXCL12 polymorphism (rs1801157) in patients with RPL

Background: Repeated abortions or Recurrent Pregnancy Loss (RPL) include a failure in continuously implantation that results in the end of pregnancy before 20 weeks of gestation. This is seen in 2-5 percent of the pregnant women. It may be caused by lack of proper blood relationship between mother and fetus occur. Many chemokines play in the development of angiogenesis, one of the most important of which is CXCL12. This protein is a polytropic chemokine that interacts with its receptor with various biochemical pathways. This ligand controls the proliferation and differentiation fate. The polymorphism, rs1801157, is located in the 3 UTR region of the CXCL12 gene, which has been identified as a risk factor for many cancers with a similar biological pathway. Materials and Methods: In this study, DNA was extracted from peripheral blood of 80 persons of RPL patients and control. This SNP (rs1801157) was evaluated by using PCR and RFLP method. The results were confirmed by sequencing. The statistical analysis was done by Chi-Square (P<0.05).Results: AA, AG and GG genotypes were observed 29%, 32% and 39% in patients respectively, whereas they were 18%, 44% and 38% in control group respectively.Conclusion: According to our results, this polymorphism (rs1801157) was not significantly correlated with susceptibility of RPL in our studied population. It seems to require more samples to express the certainty of the results.

کلیدواژه ها:

Recurrent Pregnancy Loss (RPL) ، C-X-C Motif CXCL12Chemokine Ligand 12 ، ، Polymorphism