Identification and molecular characterization of Aminopeptidase N-1 of Anopheles stephensi as a potent candidate molecule for transmission blocking vaccine (TBV) development

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 430

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شناسه ملی سند علمی:

DCME02_016

تاریخ نمایه سازی: 19 آذر 1398

چکیده مقاله:

Background: Malaria is one of the most important infectious diseases. Therefore, developing of preventive strategies is vital. Vaccines that interrupt malaria transmission (VIMTs) are one of the main solutions for global malaria eradication. One type of VIMTs are vector based vaccines that affect the sexual stage of plasmodium life cycle. One of the vector based VIMTs with acceptable blocking activity is aminopeptidase N-1 of An. gambiae. An. gambiae is the main malaria vector in Africa and Anopheles stephensi is one of the most important of malaria vectors in the world, especially in north of Africa to the southeastern of Asia.Objective: we decided to characterize this candidate molecule in Anopheles stephensi as the important malaria vector in the Middle East region.Material and method: The APN-1 mRNA sequence was characterized by 5’ genome walking and 3’ RACE. Subsequently, structural feature of the coded protein was evaluated by In silico approaches.Result: Comparing the sequences and structures of APN-1 of An. Stephensi and its counterpart in An. gambiae by Phylogenetic tree analysis and superimposition revealed that those are very similar in structurally important residues.Conclusion: Based on the structural analysis results and high similarity between APN-1 in An. stephensi and An. gambiae, it seems that APN-1 of An. stephensi could be considered as a candidate molecule for malaria vaccine development and further analysis.

نویسندگان

Javad dadgar pakdel

Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran.۲: Trauma Research Center, Sina Hospital, Tehran University of Medical Sciences, Hassan Abad Square, Imam Khomeini Avenue, Tehran, Iran,

Abbasali raz

Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran

Sedighe Zakeri

Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran

Navid Dinparast Djadid

Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran