In Vitro and In Vivo Effectiveness of Thymol against Leishmania infantum

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 353

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شناسه ملی سند علمی:

DCME02_246

تاریخ نمایه سازی: 19 آذر 1398

چکیده مقاله:

Background and Objectives: There are many limitations and adverse effects to the drugs used for treating Leishmania Infantum including pentavalent antimonial compounds and amphotericin B. for example antimoan is highly toxic, has a long treatment period and is painful in the injection site. Thymol is a monoterpene which is widely used as a bactericide, antivirus, antifungal, pesticide and acaricide.Methods: Thymol stock solution which was purchased from sigma, Germany in combination with DMD 1% was prepared and IC50 of the drug was determined and in order to determine the cytotoxicity of Thymol MTT method was used. Different concentrations of Thymol was used to inhibit Leishmania Infantum growth. Then golden hamsters were infected and histopathology assessment was performed in order to show which concentration of Thymol can be effective compared to usual drugs.Results: In the present study 10 mg/ml of Thymol had the most inhibitory effect on the growth promastigotes of Leishmania Infantum compared to control group in MTT-24hr and was comparable to 45 mg/ml of glucantime as a standard drug of choice. Also histopathological evaluation of the liver and spleen of the infected hamsters showed that Thymol was effectively active against Leishmania Infantum in vitro and human models.Conclusion: The essential oil of thymol is highly promising candidate for the development of effective and safe drugs in the fight against VL.

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نویسندگان

Mohammad Amin Ebrahimi

Graduate faculty of veterinary medicine, Islamic Azad University, Babol-Branch, Babol ۸۴۱۵۶۸۳۱۱۱, Iran

Mohammad Reza Youssefi

Department of Pathobiology, Babol-Branch, Islamic Azad University, Babol ۸۴۱۵۶۸۳۱۱۱, Iran

Seyed Mohammad Hosseini

Department of Pathobiology, Babol-Branch, Islamic Azad University, Babol ۸۴۱۵۶۸۳۱۱۱, Iran