The cardiomyocyte conversion of bone marrow-derived stromal cells (BMSCs) in 2D and 3D cell culture methods

Introduction Because of the limited potential of the heart to restore its injured area, stem cell treatment is a hopeful treatment for myocardial infarction (MI). It has been demonstrated that cultivation of bone marrow-derived stromal cells (BMSCs) with preliminary stages of cardiac lineage conversion in vitro, can have a beneficial consequence on their therapeutic potential to cure MI. Objectives On the foundation of these well-known main fact we were decided to evaluate the direct co-culture of rat BMSCs with mouse almost pure cardiomyocytes (APCSs) and cardiac niche cells (CNCs) in 2D and microfluidic chip. Methods and Results Our consequences revealed that the difference of beat rate in APCSs and CNCs in 2D and microfluidic chip during 4 weeks was not statistically significant. Obtained data from Real-time PCR study exhibited that differentiated BMSCs in all groups expressed cardiac genes of GATA4, Nkx2.5, and connexin 43, nevertheless, the expression of other cardiac specific genes such as cTnT, cTnI, and β-MHC were not noticeable through 7 days. BMSCs revealed a greater expression of these cardiac factors in microfluidic cell culture system than those cocultured in 2D cell culture. Immunocytochemistry (ICC), similarly showed that differentiated BMSCs in all groups expressed GATA4. Conclusion As a result of created shear stress, the cardiac genes expression were statistically higher in microfluidic chip than 24 well plate.

کلیدواژه ها:

Bone marrow-derived mesenchymal stromal cells (BMSCs) ، microfluidic system ، differentiation ، coculture ، cardiomyocyte isolation