Comparing Histone Modification for Transcription Factor and Chemical Induction of Fibroblast into Cardio-myocyte

Background: The potential of reprogramming of mammalian heart is limited, Majority of them fail to recover during injury and disease. Hence, reprogramming of cardiac cell from dif-ferent kind of stem and non-stem cells has a great potential for basic studies and therapeutic application and can be carry out as innovative strategies for replacing cardiac cells. Different steps were developed to regulate the expression of genes involved in reprogramming of cardiac cells. One of the steps in regulating the gene expression in cells undergo reprogramming either by a number of chemicals or transcription factors is histone modifi-cation. Modification in histones including methylation, acetyla-tion etc., play a crucial role in conversion of cardiac cells which is poorly investigated. In this study, we identified histone modi-fication for two type of induction in response to transcription factors and chemicals. For this goal, we have used two tran-scriptome datasets from direct conversion to cardiac cells by TFs and chemicals to identify the key modification of histones in either of studies and then comprising similarities/differences. Materials and Methods: In this study, differentially expressed genes with fold change equal or above 1.5 were isolated from to-tal gene expression, using R language program for both studies. Then, histone modification extracted by Enrichr online tools. Plus, the modifications of histone with highest number of score in regulatory network were extracted. Finally, we compared the modification for similarities/differences in both studies.Results: Network Analysis have showed the most important differentially expressed genes and their histone modifications during early stage of maturation of induced cardiomyocyte. Be-sides, we have introduced key histone modifications during this conversion, based on type of induction (chemical or TF).Conclusion: Our finding could be helpful to achieve regulatory elements involved in maturation pathway due to their type of induction. Also, key histone modifications would be useful in generating of more efficient induced cardiomyocytes for thera-peutic applications.

کلیدواژه ها:

Direct Conversion ، Histone Modification ، Network Analysis