Preparation and characterization of composite cellulose acetate nanofibers incorporating MOF nanoparticles as antibacterial wound dressing

Wound healing is a complicated process in which the skin, and the tissues under it, repair themselves after injury. Appropriate conditions accelerate healing process, so correct wound care is very important that can be obtained by using wound dressing. Wound dressings were applied to prevent second injuries and infections for years. Some important parameters for wound dressing design is keep moisture, gas exchange with air, Excellent oxygen diffusion and the ability of vaporize excess moisture, prevent infections by inhibiting microbial diffusion and drug release.The aim of this research is preparation and characterization of composite cellulose acetate nanofibers incorporating Metal organic framework nanoparticles as a wound dressing. This mat should have appropriate mechanical and morphological properties and also be suitable for cell growth.First, cellulose acetate nanofibers were prepared and optimum parameters were obtained. Then, the solution were blended with ZIF-8 nanoparticles and analyzed to investigate its properties. The morphology of ZIF-8 were characterized by Zeta potential, DLS, FTIR and FESEM. The characteristics of cellulose acetate mat are also characterized by swelling, FTIR, SEM and biocompatibility and antimicrobial tests. The results indicated that nanofibers diameter increased by nanoparticles addition from 276 to 423 nm. The tensile strength of nanofibers incorporated nanoparticles improved from 0.95MPa to 1.259MPa and also nanofibers strain decreased from 4.4 to 1.9. Mats swelling degree decreased from 67% to 47% because of nanoparticles hydrophobicity and decreased porosity. The antibacterial properties of the samples were investigated by both disk diffusion and CFU tests and proved that samples has 88 % and 54% inhibition for gram positive and gram negative bacteria. In addition, the prepared wound dressing has appropriate properties for cell adhesion and cell growth and all samples are biocompatible (over 90% viability).

کلیدواژه ها:

nanocomposite ، Cellulose acetate ، nanoparticle zif-8 ، Electrospinning ، Wound dressings