Mapping of sentinel lymph nodes with lymphozurrin in colorectal cancer, comparing serial sections method (H&E) with IHC (Anticytokeratin) Method for detection of micro metastasis

سال انتشار: 1392
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 364

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شناسه ملی سند علمی:

ACPLMED15_051

تاریخ نمایه سازی: 20 آبان 1397

چکیده مقاله:

Introduction:Significances and technique to use for detecting micro metastasis (MM) Isolated tumor cells(ICTs) are controversial issue among investigator. We evaluated different technique onsentinel lymph node to detect MM-ICTs.Materials and Methods:91 sentinel lymph nodes of 15 lymph node patients underwent serial section with 100μminterval. In each level two sections were prepared. One section stained with HSE and anotherstained with anti cytokeratin antibody (IHC). Then sections were evaluated for detectingMM/ICTs. Result was analyzed by chi-square test.Results:1656 section of 91 SNL of 15 patients were evaluated by pathologist and MM was find in oneand ICTs in one case, overall two out of fifteen case, and % 13/3 of patients shows MM/ICTsby IHC staining.Discussion:This study could ultra stage lymph node negative colon cancer patient 13/3% by usingcombination technique of serial section and IHC staining.In other studies with different combination of serial section, IHC staining and PCR,investigators were able to findMM/ITCs from 3% to 39% of case.In our study although ,serial section and IHC staining could ultra stage up to 13/3% ofpatients but it couldn’t affect on 5 years survival of patients.

نویسندگان

Mohammad Hossein Sanei

Isfahan University of Medical Sciences, Isfahan, Iran

Seid Abbas Tabatabie

Isfahan University of Medical Sciences, Isfahan, Iran

Seid Mozafar Hashemi

Isfahan University of Medical Sciences, Isfahan, Iran

Ali Cherei

Isfahan University of Medical Sciences, Isfahan, Iran