Apoptotic effect of organophosphorus insecticide diazinon on rat ovary and protective effect of vitamin E

Background:diazinon(DZN) is an organophosphate insecticide that widely had been utilized in agriculture all over the world and caused many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN on apoptosis of ovarian follicles in adult rats and and to assess the protective role of vitamin E. materials and methods: total of 30 adult female Wistar rats were divided into five groups: control group(without any intervention), sham group (received only pure olive oil, as solvent), experimental group 1 (DZN+olive oil, 60 mg/kg), experimental group 2 (vitamin E, 200 mg/kg), and experimental group 3 (DZN+vitamin E, the same dosage). All drugs were injected intraperitoneally, except vitamin E which was administrated by gavage. The animals were scarified after two weeks and left ovary was used to measure apoptosis of ovarian follicles. Results: The result of the present study revealed that the number apoptotic cells Diazinon group increased significantly in contrst control group in secondary and graffian follicles(p< 0.001).Administration vitamin E plus DZN, significantly reduces apoptotic cells compared to DZN group (p< 0.001).Conclusion: Results showed DZN-induced apoptosis in secondary and graffian follicles and vitamin E inhibited apoptosis induced by DZN. The results of this study suggested that vitamin E may have a protective effect on DZN-induced ovarian toxicity.

کلیدواژه ها:

Diazinon ، apoptosis ، Ovary ، Vitamin E