The effect of combination therapy with Glibenclamide and Caffeine on Malondialdehyde level in the brain of Streptozotocin-induced diabetic rats

Background and objective: Increase of oxidative stress and free radicals production in diabetes mellitus, have destructive effects on the brain. Today glibenclamide is widely used in treatment of diabetes but it can cause hypoglycemic shock. Caffeine is an antioxidant agent with high consumption in the world. This study designed to evaluate the effect of combination therapy with glibenclamide and caffeine on malondialdehyde level in the brain of streptozotocin-induced diabetic adult male rats. Materials and methods: In this experimental study, 32 male adult wistar rats ,weighing 200-250 g, were randomly divided into 4 equal groups as follows: diabetic control without treatment, diabetic treated with glibenclamide, diabetic treated with caffeine and diabetic treated with glibenclamide and caffeine. Diabetes was induced by a single intraperitoneal injection of streptozotocin (60 mg/kg). on the third day after injection of streptozotocin,the blood samples from the tail were taken and rats with a blood glucose level over 250 mg/dl were considered as diabetic rats and were used in the experiment. From the third day after diabetes induction , treated group with glibenclamide get daily 0.3cc, treated group with caffeine get daily 0.5cc and combination therapy group get daily 0.15cc glibenclamide and 0.25cc caffeine by intraperitoneal injection. Two weeks after drug injection the rats anesthesized by ether and after euthanasia, the brain tissue separated. After homogenization and centrifugation, malondialdehyde and protein measured with thiobarbituric acid and Bradford methods respectively. Malondialdehyde content of brain tissue measured as oxidative stress index with spectrophotometry method. Quantitative data analyzed with one way ANOVA with SPSS.Results: In the third day after diabetes induction, blood sampling from the tail showed that all rats became diabetic. Statistical analysis showed that treatment with glibenclamide(p=0.13), caffeine(p=0.17) and combination of glibenclamide and caffeine(p=0.13) didn’t make significant reduction in blood sugar in comparison with control group. Conclusions: Glibenclamide, caffeine and combination of glibenclamide and caffeine don’t have any effect in lipid peroxidation reduction in the brain tissue of diabetic rats.

کلیدواژه ها:

Diabetes Mellitus ، Rat ، Brain ، Oxidative Stress ، Malondialdehyde ، Glibenclamide ، Caffeine