Therapeutic effect of a combination of diazepam and glibenclamide on Catalase Activity of liver in Streptozotocin-Induced Diabetic Rats

Background and Objective: Oxidative stress caused by diabetes reflects an imbalance between the production of free radicals and antioxidant defense system .and cause biochemical alterations in the liver . Glibenclamide is commonly used in the treatment of diabetes. also Diazepam is a current drug in diabetic patients due to its sedative and anxiolytic properties .but its effects on the therapeutic role of glibenclamide is unknown.so This research aimed to investigate the therapeutic combination effect of diazepam and glibenclamide on catalase activity of liver in Streptozotocin-Induced Diabetic Rats. Materials and Methods: In this experimental study, 32 adult male Wistar rats were allocated into four groups including diabetic, diabetics treated with glibenclamide, diazepam, and diazepam-glibenclamide combination. Diabetes was induced by the injection of a single dose of 60 mg/kg of streptozotocin. To ensure diabetes, three days after injection, blood glucose levels were measured from the tail and sugar over 250mg / dl was considered as the basis of diabetic rats. The drug dose for glibenclamide was 0.3cc, for diazepam was 0.2cc daily and for their combination The concentration of both drugs was half before. Treatment continued for 16 days and at the end of the experimental period The rats were anesthetized and liver tissue expelled by opening the peritoneal space .the level of Catalase Activity was measured due to Beer and sizer method in liver homogenate. Statistically, the quantitative data obtained, compared among the groups by one-way analysis of ANOVA. Statistical significance was considered at p<0.05. Results: Treatment of diabetic group with glibenclamide non- significantly enhanced catalase activity versus untreated diabetic rats (p> 0.05) and diazepam treatment of diabetic group did not significantly change catalase activity. In addition, treatment of diabetic rats with combination of glibenclamide and diazepam non-significantly increased catalase activity versus diabetic one. (p> 0.05) Conclusion : Results showed Treatment with both glibenclamide and diazepam dosn’t have meaningful and tangible impact on the catalase activity in liver tissue

کلیدواژه ها:

Diabetes mellitus ، oxidative stress ، liver ، diazepam ، glibenclamide