preventive effects of Glucophage on intestinal ischemiareperfusioninjury in rabbit model

ack Ground: Acute intestinal ischemia is a vascular emergency that is lifethreateningand requires early diagnosis and intervention to maintain adequatemesenteric blood flow and prevent intestinal necrosis and death. The underlyingcause of this condition is variable, and its prognosis depends on its exact pathologicalfindings. Glucophage is an Antidiabetic drug that is used alone or in combinationwith other drugs to treat type 2 diabetes. The aim of this study was to evaluate theeffect of Glucophage on ischemia-reperfusion injury in rabbits.Material&Method:For this study, 15 male New Zealand rabbits in a weighing rangeof 600 ±20 gr were used. They were randomly divided into 3 groups of 5 rabbits,including surgery, ischemia-reperfusion (I/R), and ischemia-reperfusion, plusGlucophage therapy (I/R+GLO). No drug was used in surgical and I/R controlgroups. In the I/R+GLO group, Glucophage was injected for 10 days beforeinduction of ischemia-reperfusion in 200 mg/kg dose. To induce ischemia in theintestine, abdominal aorta was blocked in the upper part of the celiac artery for 30minutes by a non-traumatic vascular clamp. After removing the clamps andremoving the obstruction, reperfusion was performed for 60 minutes. Sampling fromthe intestine was performed in all groups 45 minutes after reperfusion. Finally, thedata were analyzed by one-way ANOVA at a significant level of p <0.05.Results:In zygotic studies, control of rabbits in the control group was completelyhealthy and there was no significant tissue damage in the jugular mucosa of thisgroup. In the group I/R rabbits, the tissue changes included infiltration of theinflammatory cells, a decrease in the height of the lice and ulcers Mutation wassignificantly (p =0.000) compared with the control group. In the I/R+GLO group,Glucophage significantly (p=0.000) prevented the occurrence of severe tissue damageand included only a slight decrease in the height of the pelvis, A small necrosis of theepithelial cells of the vertebral apes and focal infiltration and very few inflammatorycells in the intestinal mucosa were a number of rabbits, Thiobarbituric acid (TBA)was used to measure malondialdehyde and then absorption at 532 nm was performedby a spectrophotometer. The mean concentrations of malondialdehyde in the IRgroup were 79 ± 0.03999 and in the IR + GLO group 72.8 ± 0.03135.Conclusion:The results of this study showed that Glucophage effectively preventsintestinal ischemia-reperfusion injury

کلیدواژه ها:

Intestine ، Ischemia-Reperfusion injury ، Glucophage ، Rabbit