Histopatological study of conjanctival tissue after Plasma treatment

Purpose: Plasma offers an alternative to invasive surgical cosmetic procedures, reducing recovery time and any complications that can arise before and after surgery. Based on this topic, for the first time we evaluated the safety of the plasma on the conjactiva tissue in animal model. Methods: Twelve adult male New Zealand albino rabbits which divided into five groups were used. Experiments were performed on conjunctiva under the influence of created plasma by the Plexr device. Four spots were created on the conjunctiva using white hand piece of Plexr. For evaluation of plasma safety, ocular surface integrity and histopathological changes were assessed after 48 h (A), one week (B), one (C)and three (D) months after intervention. Results: After 48 h of plasma administration subconjunctival and conjunctival stromal edema and decreased blood vessels is noted .Epithelium reveals focally loss and marked thinning. After one week, all specimen show cojunctival sromal edema but less than group A without any congestion and hemorrhage. Epithelium show mild repair. Decreased blood vessels in the stroma is also noted. Generally, after one month loss of integrity of corneal epithelium has been completely improved. There is no evidence of malignancy in conjunctiva after 3 months. Conclusion: Our results showed the safety of Plasma on the conjunctiva in animal model. Plasma may be an alternative method for treatment of some ocular surface disorders. For more reliable results, long term study in animal model and a pilot clinical trial in human is proposed.