Immunohistocehmical analaysis of MBP and PDGF-α receptor after After Mesenchymal Stem cell Injection in Cuprizone Model of Multiple Sclerosis

Objectives:Multiple sclerosis (MS) is one of the most important inflammatory diseases of the central nervous system, yet its etiology is not well defined. Cell therapy for MS has become one of the newest therapies, even in its clinical phase. Material and Method: First, human bone marrow stromal stem cells are cultured with α-MEM containing 10% FBS. Demyelination model was induced in mice for 6 weeks by administration of the cuprizone, and in the fourth and fifth week after the first dose of cuprizone, the stem cells were administered intraperitoneally to the mice. At the end of the sixth week, the mice were killed and subjected to tissue evaluation using the LFB staining technique for myelin pods also MBP and PDGF-α receptor immunohistochemistry. Results: In the Immunohistochemistry technique for MBP and PDGF-αR increased recovery in the treatment group with the cell was seen compared to other groups. Conclusion: In groups where we used stem cell, we observed an increase in myelination compared to other groups that we used only from PBS.

کلیدواژه ها:

Stem cell ، Mesenchymal ، Cuprizone ، Myelination