Cytotoxic Effect of Hydroalcholic Extract from Thymus daenesis Celak on MCF-7 Cancer Cells Line
محل انتشار: اولین کنفرانس ملی یافته های نوین زیست شناسی
سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 333
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شناسه ملی سند علمی:
BIOC01_268
تاریخ نمایه سازی: 11 خرداد 1397
چکیده مقاله:
Breast cancer is the second most common cancer in women after lung cancer. Given that the herbal ingredients are used for centuries to treat cancer, The aim of this study was to determine the cytotoxic effect of hydroalcholic extract from Thymus daenesis Celak on MCF-7 cancer cells line.Breast cancer cells MCF-7 and natural fibroblast cells were cultured in DMEM medium containing fetal bovine serum and antibiotics. The cells were exposed to different doses of hydroalcholic extract of Thymus daenesis Celak (0.156, 0.312, 0.625, 1.25, 2.5 mg/ml) and incubated for 24, 48 and 72 hours, respectively. After incubation, the modified MTT colorimetric test was used to determine cytotoxicity. The results of MTT test showed that hydroalcholic extract of Thymus daenesis Celak has dose- and time-dependent anti-cancer effect on MCF-7 cancer cells, so that by increasing the concentration and 72h incubation, the most cell death was observed (P<0.05). Plant extract did not show significant cytotoxicity on natural fibroblast cells. Then, it seems that its compounds can be used in treating cancer through more future .research
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نویسندگان
Fatemeh Sadeghi Samani
MSc of animal Physiology, Department of biology, Faculty of Basic Sciences , Shahrekord Branch, Islamic Azad University, Sahrekord, Iran
Hoseein Sazegar
Assistant professor, Department of biology, Faculty of Basic Sciences , Shahrekord Branch, Islamic Azad University, Sahrekord, Iran
Abdollah Ghasemi Pirbalouti
Associate professor, Department of Medicinal Plants, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran