Study of the differentiation of rat omentum stem cells to nerve cells using brain tissue extract of rat

Stem cells have two typical feature self-renewal and potential of producing different cell lineages. Omentumstem cells could be induced to differentiate into neural cells under certain condition. The main goal of this present study is to neural differentiation of OSCs induced by using Rat brain extract. Omentum stem cells were isolated from peritoneum and brain extract from neonatal rat brain, then the cells were cocultured in DMEM and brain extract. After 3 passage OSCs were morphologically observed and processed for RT-PCR. The number of cells neural morphology increased at 1-2 weeks dramatically. The cells were labeled by neural markers including Map2 which assessed by immunocytochemistry. Our finding demonstrated that OSCs efficiently differentiate into the neural cell when cultured in brain extract

کلیدواژه ها:

Omentum ، Mesenchymal stem cells ، Rat neonate brain extract ، Neural cell differentiation