Optimization of exopolysaccharide production by Bacillus Sp.IR12 in different culture media

Exopolysaccharides are a natural component that produces by various kind of bacteria and they have many applications in the food industry. A consolidating and stabilizer role for color and taste of foods and in the pharmaceutical industry. They decrease blood cholesterol and triglyceride and their anti-cancer effects have been emphasized. In this research exopolysaccharide production by Bacillus Sp.IR12 has been done by a liquid culture medium that contains magnesium sulfate, ferrous sulfate, chloride ammonium and D-potassium phosphate, glucose and salt. We study designed test by Taguchi 9.test under 3 variable of temperature 28, 30, 32, time by 48, 72, 96 degrees and salt density 1%, 2%, and 3% and at the end results had been analysis by Quality-4. Results show that by time factor intervention maximum production rate was exopolysaccharide at 32 that was equal to 66/4, but for time factor maximum of exopolysaccharide production was 72 hours equal to 85/4% and for salt density factor we observed that maximum exopolysaccharide production was at a salt density of 3% and equal to 35/5%. General results show that one of these factor effects on optimization of exopolysaccharide production depends on other factors and this bacterium has a good potential for exopolysaccharide production and we can say that this bacterium is the proper choice for industrial usage

کلیدواژه ها:

Exopolysaccharides ، Taguchi method ، Optimization ، Bacillus