Tannase production by Trichoderma species in tannin containing medium as a sole carbon source

The aim of this study was an evaluation of tannase production by Trichoderma viride (Tv), Trichoderma harzianum (Th), Trichoderma longibrachiatum (Tl), and Trichoderma reesei (Tr) in the medium containing tannin as a sole carbon source. Trichoderma species were cultured in Trichoderma fermentation medium (TFM) which contained 1% Tanic acid solution in 0.2 M acetate buffer (pH=5.5) for 5d. Tannase activity determined by addition of 0.5 mL of culture supernatant (crude enzyme) to 2.0 ml of 0.35% (w/v) tannic acid solution in 0.05 M citrate buffer (pH 5.5) in a test tube at 40 ºC. The absorbance was measured at 310 nm. The protein content in the TFM supernatant after 48 h fermentation was estimated by the dye binding method of Bradford. The level of protein produced in the medium by Trichoderma viride was lower (P<0.05) than the other species. There were no differences in enzyme production and enzyme specific activity between Trichoderma species. The specific activity of tannase for Tv, Th, Tl, and Tr were 43.00, 48.50, 66.50 and 58.00 U/ml, respectively.

کلیدواژه ها:

Trichoderma ، Tannase ، Tannin ، Enzyme activity