Immobilization of urease enzyme on carbon nanotube and iron nanoparticles with comparison of their physiochemical features

Urease enzyme is widely used in various industries and medicine. Increase the physicochemical properties of this enzyme can make it easier to use in various fields. Different nanomaterials are now used to immobilize enzymes. Carbon nanotubes and iron nanoparticles are among the most commonly used nanomaterials to immobilize enzymes that give them unique properties. In this study, the urease enzyme was extracted from red beans and then immobilized physically on carbon nanotubes and iron nanoparticles by covalent bonds. The temperature and magnetic properties of the enzyme after the immobilization were investigated, indicating an increase in thermal resistance after immobilization on carbon nanotubes. Also, enzyme activity was reduced by immobilization on each of nanomaterials. Finally, the fixation of the enzyme on iron nanoparticles can lead to multiple uses of the dialysis device. Also, at higher temperatures, the enzyme inactivity decreases

کلیدواژه ها:

Enzyme ، Immobilization ، Urease ، Carbon nanotube ، Iron nanoparticles