Spectroscopic investigation of groove binding interaction of Fe3O4@CaAl LDH@L-Dopa with Calf Thymus DNA

The main goal of this study is the evaluation of groove binding interaction of Fe3O4@CaAl LDH@L-Dopa with calf thymus DNA (CT-DNA). The magnetic nanoparticles were prepared by a chemical co-precipitation method and the surface of the Fe3O4 nanoparticles was coated with CaAl layered double hydroxides (CaAl LDHs). This coreshell nanostructure was used as a carrier for the antiparkinsonian drug L-Dopa to achieve the drug delivery system with suitable properties for biological applications. The structural features of Fe3O4@CaAl LDH@L-Dopa were evaluated using various techniques like XRD, FT-IR, TEM, and SEM. According to the obtained results from the physicochemical analysis, the core-shell structure of Fe3O4@CaAl LDH@L-Dopa with about 120 nm average size, was confirmed. Also, the interaction of Fe3O4@CaAl LDH@L-Dopa with CTDNA has been studied using, UV-Visible spectroscopy, viscosity, circular dichroism (CD) and fluorescence spectroscopy techniques. The results of investigations clearly demonstrated that Fe3O4@CaAl LDH@L-Dopa has interacted via groove binding to CT-DNA

کلیدواژه ها:

L-Dopa ، Layered double hydroxide ، Magnetic nanocarrier ، Drug delivery ، Drug release ، Cell culture