Loading Jellyfish venom C-CfTXA-STxB chimeric antigen in PLA-PEG-PLA copolymers with a tri-block configuration

The venom of C. fleckeri box jellyfish contains a variety of bioactive proteins that are cytolytic, cytotoxic, inflammatory or lethal. Poly Lactic Acid is a biodegradable and biocompatible polymer approved by the FDA. Adding PEG to PLA lowers the zeta potential, yields a higher uptake and does not absorb plasma proteins, resulting in a higher shelf life in the bloodstream. The cytolytic C.fleckeri toxin C-CfTX1-STxB chimeric recombinant protein was produced by the research group in the laboratory previously. The aim of this study was to Nanoencapsulate above-mentioned chimeric product in PLA-PEG-PLA tri-block purchased copolymer. To investigate the increase in the potential of action after nanoencapsulation by double emulsion solvent evaporation techniques, acetone, due to the low boiling point, easy removal and less toxicity was selected as a solvent. The antigenicity potency of encapsulated C-CFTX1-STXB was compared with the naked one.Based on the results, the efficiency of encapsulating C-CfTX1-STxB in PLA-PEGPLA nanoparticles was approximately 71%. The Appearance of the nanoparticles was studied under SEM revealed an apparent uniformity in the dimensions below100 nm, spherical and smooth surface with a Proper density. DLS was employed to evaluate the particle size. The data obtained from the study of the hydrodynamic radius and the electrical potential of the particle surface (zeta potential) also indicate that the nanoparticles are in a stable range.The release of C-CfTX1-STxB reached 49.6% after 30 days.Encapsulated and naked antigen and PBS were injected into mice, then the antibody titer in their serum was measured by ELISA and its quality was measured by Western Blot. Considering that recombinant C-CfTX1-STxB protein doesn t have cardiotoxicity and neurotoxicity effects on mice, and the high homology of selected segment and due to controlled and slow release in copolymer this produced protein can be used as a candidate for a vaccine against Jellyfish venom

کلیدواژه ها:

Chironex fleckeri ، C-CFTX1-STxB chimeric antigen ، PLA-PEG-PLA tri-block copolymers ، Nanoencapsulation