Studies on the interaction of the drug Indinavir with calf thymus DNA by resonance light scattering and circular dichroism spectroscopy
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 296
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شناسه ملی سند علمی:
BIOCONF20_429
تاریخ نمایه سازی: 28 اردیبهشت 1398
چکیده مقاله:
The molecular DNA contains all the information necessary for theDevelopment and biological functioning of the living organisms and the virus.DNA is one of the biological macromolecules that plays a role in the transmission of genetic information. This macromolecule is a long polymer of simple building blocks called nucleotides And according to Watson & Creek Model, DNA is Right-handed double-helix with a major and a minor grooveSo that The organic bases are located inside the helix and the sugar-phosphate backbone on the outside and connection of the sugar and the phosphate groups is carried out through the ester bond.Bases that are opposite each other are paired according to defined rules as a result of hydrogen bonds. Indinavir is a viral protease inhibitor used to treat HIV and results in the release of abnormal and non-infectious viruses. In this study, the interaction between Indinavir drug and calf thymus DNA at ambient temperature and pH=6.8 was investigated by resonance light scattering and circular dichroism spectroscopy. According to the results arise from resonance light scattering we conclude that a change in the macromolecular structure, which confirms the formation of a complex between the Indinavir and the calf thymus DNA. Thechart Critical Point of Creation ofSediment in the presence of increasing amounts of indinavir, in addition to the emphasis on the formation of a complex between drug and DNA, was used to determine the critical concentration of sediment generation. Cd spectroscopy was used to study the structural changes in DNA in interaction with Indinavir the results of the cd spectra showed that the interaction of Indinavir with DNA caused a change in the structure of the second DNA. Also, according to the results obtained, it is likely that this interaction occurs between the base pair of DNA
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نویسندگان
Ladan Shakoorzadeh Ardebili
epartment of Biochemistry and Biophysics, Faculty of Sciences,University of Islamic Azad, Mashhad Branch, Iran
Mohammad Reza Saberi
Department of Medicinal chemistry, Faculty of Pharmacie, University of Ferdosi, Mashhad Branch, Iran
Jamshid Khan Chamani
epartment of Biochemistry and Biophysics, Faculty of Sciences,University of Islamic Azad, Mashhad Branch, Iran