Expression of miR-7, miR-409 and miR-93 in patients with colorectal cancer who referred to Tehran hospitals by Real Time PCR

Neoplasm including colorectal cancer is often diagnosed in the late stages and is dumped with weak prognosis. Although tumor markers greatly improve diagnosis, but there is numerous problem yet because of the nature of invasive, inconvenient and troublesome of the current methods. Hence, there is an urgent need to identify noninvasive biomarkers for early detection of cancers. Also, recently the changes in miRNA expression in various cancers have been reported. Present study was designed to investigate the relationship between the expression of miR and colorectal cancer. The expression level of miR-7, miR-409 and miR-93 in patients with colorectal cancer and control groups was assessed by Real Time PCR.The study was included 30 patients with colorectal cancer and 30 healthy individuals with no history of colorectal disease. The age and conditions of the patient group was conducted. In order to examine the expression level of miR-7, miR-409 and miR-93 in patients with colorectal cancer and healthy people qRT-PCR techniques were used. First, RNA was extracted from plasma samples and finally, using the qRT-PCR, expression levels of miRs were measured and compared with the control group. By doing statistical analysis (t-Student Test) changes of miRs expression in patients and controls group was analyzed.After statistical analysis, results showed that the expression levels of miR-7 and miR-409 in the plasma of patients with colorectal cancer was significantly higher than the controls group, 4.3 and 4.2-fold respectively. Also did not observed significant change between patients and controls group in expression levels of miR-93.The results showed that over-expression of miR-7 and miR-409 in plasma may be associated with colorectal cancer. These miRs probably have had the potential to use as the noninvasive diagnostic markers to detect colorectal cancer

کلیدواژه ها:

Non-Invasive Biomarker ، Rapid Diagnostic Test ، miRNA