Expression analysis of Long non-coding RNA SNHG17 in breast cancer

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 284

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شناسه ملی سند علمی:

BIOCONF20_483

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Breast cancer is a common type of cancer among women worldwide. In recent decades despite of impressive advances in diagnostic and therapeutic strategies, mortality of breast cancer is still significant. Studies demonstrated that Long non-coding RNAs (lncRNAs) as an important group of non-coding RNAs play key roles in development and progression of different cancers, including breast cancer. SNHG17 (Small nucleolar RNA host gene 17) is a novel lncRNA that have been reported in some cancers such colorectal cancer. This study aimed to evaluate the expression of SNHG17 in breast cancer. Breast tumor tissues and their non-tumoral marginal samples were obtained from 30 patients with breast cancer from the Nejat hospital of Tabriz between 2014 and 2015.Demographical data like age, grading of the samples and were collected. Total RNA was purified with RNXPlus and cDNA was synthesized by PrimeScript™ RT reagent Kit )TaKaRa(, then expression of lncRNA SNHG17 was quantified using qRT-PCR. GAPDH was used as internal control gene. The qRT-PCR results indicated that SNHG17 expression in tumor tissues was rather lower than to margin tissues. However, SNHG17 expression did not show correlation with demographics of patients

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نویسندگان

Saeid Amirian-ghatar

Department of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran

Mohammad Khalaj-kondori

Department of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran

Mina Zafarpiran,

Department of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran

Mohammadali Hosseinporfeizi

Department of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran