Application of bioinformatics in the design of anti-VEGF peptide

New blood vessel formation (angiogenesis) is fundamental to tumor growth, invasion, and metastatic dissemination. The vascular endothelial growth factor (VEGF) signaling pathway plays pivotal roles in regulating tumor angiogenesis.VEGF-A shows prominent activity with vascular endothelial cells, primarily through its interactions with the VEGFR-1 and VEGFR-2 receptors found prominently on the endothelial cell membrane.The aim of this study was the design of an anti-VEGF-A peptide based on VEGFR2 binding regions.The effective amino acid sequences in the interaction of VEGF-A and VEGFR-2 were investigated by using the valid bioinformatics software including Chimera and SPDBV and the binding sites of the VEGF-A molecule on the receptor were identified. These areas were considered as the basis for peptide design.In the next step, the sequence was mutated in the binding regions.The binding ability and peptide tendency were then analyzed using HadDock, Hex and ClusPro software. The bioinformatics analysis showed that the CDR3 region of the receptor played a major role in binding to VEGF-A And the peptide derived from this region after a computerized mutation, has the ability to bind with a greater tendency than VEGFR-2 to target the molecule. The present study showed that anti-angiogenesis peptide design is an effective method for inhibiting cancer growth.

کلیدواژه ها:

VEGF-A ، VEGFR-2 ، Angiogenesis