Histopathological evaluation of liver and spleen after immunized mice with recombinant PilQ, b-flagellin vaccine

Pseudomonas aeruginosa is an opportunistic pathogen that caused nosocomial infection in compromised patients. The aim of this study was to determine the histopathological changes in the liver and spleen of infected mice after treatment with recombinant PilQ and type b-flagellin antibodies in the burn mouse model. In the present study, the BALB/c mice were infected by Pseudomonas aeruginosa and then treated with antibodies raised against PilQ and type b-flagellin in different groups. The mice were sacrificed and the spleen and liver were aseptically removed. For investigation of the histopathological changes, the Hematoxylin and Eosin staining wasperformed. Studies showed that when mice were treated with anti-PilQ and type b-flagellin antibodies, reducing necrosis and hemorrhage, the reduction of inflammatory cells among hepatocyte cells and concentrations around the central vein was observed. In this group, there was an increase in megakaryocyte and decreased inflammatory cells and low changes in the red and white pulp of the spleen. In the tissue sections of the treated group with Pseudomonas aeruginosa suspension, changes in the white and red pulp occurred, and also inflammatory cells were increased compared to test groups. These results showed that co-administration of antibodies raised against recombinant PilQ and b-flagellin in the challenged mice could make less histopathological changes in the liver and spleen of mice compared to other treated groups.

کلیدواژه ها:

Pseudomonas aeruginosa ، PilQ ، b-flagellin ، Nosocomial infection