Exogenous application effect of spermine on ascorbate and glutathione pool changes in three wheat cultivar (Triticum aestivum L.)flag leaf under well-watered and droughtcondition in grain filling stage

In order to study effect of exogenous application of spermine on antioxidant potential, the experiment based on the randomized complete block design with four replications was designed for the effect study of spermine (25 mg l-1) on non-enzymatic antioxidant pool content related to oxidative stress traits changes in three wheat cultivars (Triticum aestivum L.) different from the direction of tolerance to drought stress grown in greenhouse under well-watered (100% field capacity) and drought (50% field capacity) condition starting from spike formation until its maturity. Results showed drought significantly alleviated total ascorbate of drought tolerant (Pishtaz) cultivar and dehydroascorbate and oxidized glutathione in drought tolerant and semi-drought tolerant (Sabalan) cultivars. Also, malondialdehyde and hydrogen peroxide content increased in susceptible cultivar (Gaspard) more than two other cultivars, significantly. Although spermine application had no significant effect on total ascorbate and glutathione content in studied wheat cultivars under 100 and 50 percent field capacity, it increased dehydroascorbate and oxidized glutathione content in Sabalan and Pishtaz cultivars flag leaf, significantly. Also, spermine caused the significant amelioration of malondialdehyde more than hydrogen peroxide in the tolerant cultivar, especially. Theinvestigation results showed although the exogenous application of spermine did not cause to significant effect on total ascorbate and glutathione pool, it decreased reduced ascorbate/dehydroascorbate and reduced glutathione/oxidized glutathione ratio. Tolerant cultivar (Pishtaz) benefited from the exogenous application of spermine treatment in related to non-enzymatic pool improvement.

کلیدواژه ها:

Field capacity ، Hormonal treatment ، Hydrogen peroxide ، Malondialdehyde ، Non-enzymatic antioxidant