Somatic embryogenesis induction from hypocotyl explants of Catharanthus roseus (L.) G. Don
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 474
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شناسه ملی سند علمی:
BIOCONF20_586
تاریخ نمایه سازی: 28 اردیبهشت 1398
چکیده مقاله:
The current research was conducted to simulate the efficient in vitro somatic embryogenesis of Periwinkle Catharanthus roseus. 1 mg/l 2,4-D+0/5 mg/l BAP significantly affected the calli fresh weight. 1 and 0/5 mg/l 2,4-D concentration had meaningful effect on the production of pre-embryonic tissue and subsequent embryogenesis. With the second experiment, radicle, hypocotyl and leaf explants from in vitro derived plants were employed. For callogenesis, the interaction effects of radicle × 2,4-D, hypocotyl × 2,4-D and radicle × NAA were significant. For calli fresh weight, hypocotyl × NAA and hypocotyl × 1mg/l NAA+ 1mg/l BAP were the treatments of choice. For pre-embryonic tissue and the embryos produced, hypocotyl × 2, 4-D was the best treatment.
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نویسندگان
Habib Farhadi,
Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh
Mohammad Bagher Hasanpouraghdam
Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh
Mohammad Ali Aazami
Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh