Determination of the optimal 2,4-D concentration for callus induction from different explants of Salvia tebesana Bunge

Salvia tebesana Bunge as a species of Lamiaceae is a plant with many metabolites including monoterpenes, diterpenes, sesquiterpenes, various flavonoids and tannins (with medicinal properties). The tissue culture and subsequent callus production is a way to produce and increase plant secondary metabolites. The aim of this study was the investigation of callus induction rate from apex meristem , rootstock meristem , leaf and petiole explants on MS medium with different 2,4-D concentrations (0, 0.5, 1, 1.5, 2 mg l-1 ) and finally, the determination of the optimal concentration of this hormone for inducing callus in order to subsequent use in the production of secondary metabolites. Affected samples by mentioned concentrations of 2,4-D on the MS medium were evaluated at the end of the 8th week in terms of induction rate, form and fresh weight of callus. Based on the results, apex meristem and petiole explants showed a better response to callus induction ( rootstock meristem and leaf explants were not able to produce callus). Among the investigated hormonal concentrations, 1.5 mg l-1 2,4-D had a better effect on the induction and growth of callus

کلیدواژه ها:

Salvia tebesana ، Callus induction rate ، 2 ، 4-D