Effect of plant growth regulators on micropropagation of jujube medicinal plant

The jujube tree is considered one of the most important medicinal plants due to its valuable medicinal properties, such as purifying blood and soothing the nerves. The reproduction rate of this plant is low through traditional methods, and the use of tissue culture techniques can increase reproduction and produce plants that are consistent and lack of contamination. The study investigated the effects of different treatments of plant hormones on the amount of regeneration and proliferation of jujube. Stem samples of jujube trees of South Khorasan were used as explant in May and June. Ethanol (70%) and sodium hypochlorite (10%) were used for sterilization. Samples were cultured in 6 different hormonal treatments containing IBA (0.05, 0.1, 0.2 mg/l) and BAP (0.5, 1, 2 mg/l) in MS medium and analyzed by SPSS software using randomized block design. The studied traits were the mean of length and number of stem, branch, and number of nodes. The results comparing the means of the evaluated traits showed a significant difference at the 0.05probability level between different hormonal treatments compared to the control treatment . In the treatment containing hormones, IBA (0.05 mg/l) + BAP (2 mg/l) was observed the highest mean number of stems and nodes and the medium containing hormones IBA (0.2 mg/L) + BAP (2 mg/l) showed the highest mean shoot length. The highest number of stem branching was in the medium IBA (0.05 mg/l) + BAP (0.5 mg/l).

کلیدواژه ها:

Ziziphus jujuba ، In vitro culture ، Plant growth regulators ، Proliferation