Role of Hydrogen peroxide in the biosynthesis of Podophyllotoxin inCell Culture of Linum album

Linum album accumulate Podophyllotoxin (PTOX), with antiviral and anti-cancer properties, is used as a precursor for the semi-synthesis of established cancer therapeutics such as etoposide, teniposide and etopophos. Reactive oxygen species are toxic molecules that can react with vital molecules such as proteins, nucleic acids, lipids, and carbohydrates and cause damage to them whether Hydrogen peroxide (H2O2) plays an important role as a key signaling molecule in response to various stimuli and is involved in the accumulation of secondary metabolites. The aim was to study the relationship between PTOX accumulation and H2O2 production. Using H2O2 scavenger, dimethylthiourea (DMTU), we investigated the role of H2O2 in the induction of PTOX accumulation. In single treatment experiments, sterile DMTU in concentrations (0, 50, 100, 250 μM) were added to 7-dayold cell cultures and were harvested after 2 days. They were separated from medium by filtration under suction, weighted and immediately frozen in liquid nitrogen. The results showed that Minimum PTOX production was achieved by the addition of 100 μM DMTU 4.08 μg/g FW in compared to control 7.3 μg/g FW. This results suggested accumulation PTOX strongly decreased by DMTU and this compound increased PAL activity, flavonoide and phenol contents in cell culture linum album. Taken together, these findings suggest that DMTU is scavenger H2O2 and it appears H2O2 up-regulates accumulation PTOX in cell culture of Linum album

کلیدواژه ها:

Podophyllotoxin ، Dimethylthiourea ، Hydrogen peroxide ، Linum album