Effects of mechanical vibration on growth and some metabolic changes in Matricaria chamomilla L.

Mechanical vibration is one of an abiotic stress which can affect plant cell growth and development. German chamomile (Matricaria chamomilla) is a medicinal plant of the Asteraceae family and is widely used in the pharmaceutical industries. In this study, the seeds were sterilized and cultured in a Murashige and Skoog (MS). For callus induction, leaf explants were placed in MS medium supplemented with 2, 4-D (1 mgL-1) and Kin (1 mg L-1). After subculture, different frequencies of mechanical vibration (0, 5, 10 and 15 Hz) were applied. Results showed that mechanical vibrations at 5 and 10 Hz reduced the protein content and increased this parameter at 15 Hz frequency comparing to the control. The content of photosynthetic pigments (total chlorophyll, Chla, Chlb, and carotenoids) showed a decreasing trend at 5 and 10 Hz mechanical vibration. Although, the pigment content increased slightly at 15 Hz, but showed a decrease compared to the control. It seems that vibration at lower frequency has more destructive effects than the 15 Hz frequency, which can be related to the production of free radicals and induction of oxidative stress in cells under stress.

کلیدواژه ها:

German Chamomile ، Callus tissue ، Protein ، Mechanical vibrations