Study of expression of PMP genes and dehidrin in salt stress resistance in oilseed sunflower

Non-biotic stresses, especially salinity, have a very important contribution in reducing the yield of plants like a sunflower. The sunflower (Helianthus annuus L.) is from the Asteraceae family and it has three types: oily, honey and ornamental. In order to study the effect of salinity stress on the expression of PMP and dehydrin genes, two different lines of susceptible (CSA39) and resistant (AS5305) oilseed sunflowers were selected. Genotypes were studied in a factorial arrangement with a completely randomized design with 3 replications and 5 salinity levels (2, 5, 8, 14 dS) and 6 sampling times, in potted conditions. Stress was applied at the 8-leaf stage. Sampling was carried out at 0, 6, 12, 24 and 48 hours after application of stress using liquid nitrogen. Expression of the genes was evaluated using Real-Time PCR. The results show that the expression of the studied genes in the sensitive and resistant lines is different and the level of PMP and dehydrin expression in resistant line (AS5305) was significantly increased

کلیدواژه ها:

Oilseed sunflower ، Salinity stress ، Real-time PCR