Study of the effect of histidine amino acid on chlorophyll content, membrane stability index and celatine enzyme activity of rapeseed under heavy metal tension of lead

Today, environmental pollutants such as heavy metals, rare elements, organic compounds and radioactive substances are considered serious threats. At present, the need to prevent the spread of these contaminations and also the cleaning of contaminated areas is highly demanded. One of these methods is a plan that uses plants and microorganisms to clean contaminated environments (soil and water). For this purpose, this paper examines the role of histidine amino acid against stress induced by lead. In this study, effects of histidine concentrations (300.0 μmol/L) and lead (60.5.0 μmol/L) and Pb (NO3)2 on total chlorophyll, b and a membrane stability index and catalase activity were investigate in a completely randomized design with 3 replications in the greenhouse of Kerman Industrial Advanced Industrial University. The results of statistical analysis showed that 5 and 60 μm lead treatment significantly reduced the total chlorophyll, b and a, and membrane stability index. Also, a significant increase in the activity of the catalase enzyme was seen. The use of 300 μM of histidine alone increased the content of chlorophyll content and membrane stability index. Also, the use of 300 μm of histidine reduced the effects of lead and increased all parameters except the activity of catalase enzyme compared to 5 and 60 μm treated plants. According to the findings of this study, it seems that the effect of amino acid histidine is due to the improvement of the content of photosynthetic pigments and the membrane stability index. Also, in the case of plant-induced stress, the catalase enzyme increases the resistance of the plant.

کلیدواژه ها:

Histidine ، Chlorophyll ، Membrane stability index ، Catalase