The study of the expression of RAV gene family members in two native bread wheat (Triticum aestivum L.) landraces under salt stress

Bread wheat (Triticum aestivum L.) is one of the most important crops in the world, providing the calories needed for about 30% of the world s population. Livestock plants are subjected to various biotic and abiotic stresses. Salinity is one of the most important limiting factors in plant production among abiotic stresses. Under salt stress, wheat production is reduced by up to 50%. Different mechanisms and factors, including transcription factors, are activated to deal with abiotic stresses depending on plant and tension. One of these transcription factors is the RAV gene family that is involved in a wide range of biotic and abiotic stresses. In this study, two native wheat landraces, 3625 and 3623, were planted in 98 pot diameter of 7 cm. In the three-leaf stage pots were watered by Johnson solution contains 250 mM sodium chloride at zero (control), 1, 3, 6, 12, 24 hours and 10 days. Plant specimens including aerial part and root were collected after applying stress and immediately stored in frozen liquid nitrogen and kept at -70 ° C. In wheat, the family of the RAV gene, which is a subset of the larger family of AP2 / ERF, has been registered in 8 genes encoding 6 different proteins. Therefore, NCBI and PlantTFBD websites were used to find AP2 / ERF family members‟ sequences. On the PlantTFBD website, genes are grouped in which the genes were downloaded. Also, several genes and proteins were found on the NCBI site, which by replicated BLASTp and BLASTn all genes associated with AP2 / ERF families were collected. Then, using multiple alignments, repeated sequences were deleted. After matching the sequences in the unique regions, the primer design was performed and clones were sent to sequencing after isolation of the genes and cloning them into a suitable vector. Following the sequencing and validation of genes based on BLASTX and PLANTENSEMBL website, which confirming sequences and genes based on domains, primer designing was performed for Real-Time RT-PCR and the expression of RAV genes in the aerial parts and roots of two cultivars at different times under salt treatment was studied. The results indicated changes in the expression of a number of genes in the experimental conditions.

کلیدواژه ها:

Bread wheat ، RAV transcriptional factors ، Salt stress ، Gene expression