Nucleotide diversity in 5 cpDNA regions in Fritillaria (Liliaceae) in Iran

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 289

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شناسه ملی سند علمی:

BIOCONF20_828

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Nucleotide sequences of different regions of cpDNA are used for phylogenetic reconstruction, phylogeographic studies, and DNA barcoding. In this study sequences of 5 regions rbcL, rpl16, trnH-psbA, trnL-F, and ycf1b from 21 species of Fritillaria in Iran are used for phylogenetic reconstruction in subgen. Fritillaria and assessing nucleotide diversity and identifying a possible DNA barcode. All sequences of ycf1b and most of the trnL-F regions are new, and about half of the sequences for other regions are from gene-bank. rbcL and rpl16 regions are sequenced on both strands, and other regions are only from sense strands. Chromatograms were edited using Chromas and aligned using the MEGA7 software. Nucleotide diversity in all 5 regions was low and provided little polymorphic sites. Phylogenetic analysis of data matrix separated main groups within subgen Fritillaria (sects Olostyleae and Trichostyleae, and groups caucasica and crassifolia), but the resolution within groups was low. Results demonstrated a low evolutionary rate in cpDNA of this genus and the need for new regions to be analyzed in the nucleus or ribosomal DNA. Results of our phylogenetic analyses (NJ, ME, UPGMA) is presented as a species clustering dendrograms.

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نویسندگان

Mahsa Poodineh

Department of Biology, Faculty of Science, Shahrekord University

Majid Sharifi-Tehrani

Department of Biology, Faculty of Science, Shahrekord University

Mahfouz Advay

Department of Biology, Faculty of Science, University of Tehran