Antitumor effect of Aspirin on WNT signalling pathways in colorectal cancer cell line

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 437

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شناسه ملی سند علمی:

CANCERMED04_025

تاریخ نمایه سازی: 17 مرداد 1398

چکیده مقاله:

Introduction and aim. Nonsteroidal anti-inflammatory drugs (NSAIDs) such as Aspirin, with anti-inflammatory and anti-cancer properties can be effective as a novel strategy for the treatment of colorectal cancer (CRC). The aim of present study is to assess the antitumor effects of Aspirin on WNT messaging pathways in colorectal cancer cell line in vitro. Methods. cell viability was assessing after treatment of SW742 cells with various concentrations Aspirin by MTT assay and the amount of IC50 was determined. The rate of expression of genes on WNT pathways was also evaluated via Real Time PCR. Statistical analysis was carry out through one-way ANOVA and Tukey multiple range analysis (SPSS 19.0 software (P <0.05). Results. The analysis of the results demonstrated that Aspirin significantly inhibited the proliferation of SW742 cells. Expression of genes revealed a decrease in the expression of WNT, AXIN, TCF, APC genes and increased expression of β-catenin gene in the WNT signaling pathway. Conclusion. Our findings reveal that Aspirin inhibits the proliferation in the human colorectal cancer cell line SW742. Also, Aspirin inhibits signaling pathways of WNT in CRC cell line.

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نویسندگان

Malihe Bagheri

Molecular and Medicine Research Centre, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.

Zahra Aghasi

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Next to Milad Tower,Tehran, Iran

AmirReza Hesari

Molecular and Medicine Research Centre, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.

Faezeh Ghasemi

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Next to Milad Tower,Tehran, Iran