GPC3 expression at the mRNA level in the colorectal cancer

Introduction & Aim: Glypican-3 (GPC3) is a membrane heparan sulfate proteoglycan. It overexpresses in several types of cancer such as hepatocellular carcinoma (HCC), melanoma and etc. In the present study, the gene expression of GPC3 was examined in the colorectal cancer tissues and also in the normal adjacent tissues. Methods: In this study, the expression of the GPC3 at the mRNA level were investigated in 61 human colorectal cancer (CRC) tissues and 61 adjacent non-tumor tissues. The total RNA was isolated from the fresh frozen tissues by TRIzol reagent and cDNA was synthesized by cDNA synthesis kit. quantitative real-time PCR (qRT-PCR) was performed in an Exicycler™ 96 machine. The specific primer sequences for the GPC3 gene and the β-actin gene (as a reffrence gene), were designed using the Gene Runner software. The relative fold change in the expression was then calculated using the comparative 2−ΔΔCT formula. Results: We revealed a significant increase in GPC3 gene expression in 30 cases of 61 cases (49.18%) compared with matched adjacent tissues. There was a significant correlation between mRNA expression and pathological differentiation and a positive significant correlation between age of patients. We also, found significant reduction of the mRNA expression in 11 cases of 16 cases who underwent neodjuvant chemotherapy when it was compared with cases without chemotherapy. Conclusion: Our results indicated that GPC3 could be expressed in the CRC tissues at the mRNA level.

کلیدواژه ها:

Glypican–3 ، Colorectal cancer ، Neoadjuvant chemotherapy