Correlation of lncRNA PVT1 Upregulation with advanced stages and invasion in Esophageal Squamous Cell Carcinoma (ESCC) patients.

Introduction & Aim: Esophageal Cancer (EC) as a lethal malignancy with lower survival rate is considered as an incurable disease. Abnormal expression of long non-coding RNAs in cellular processes related to EC have extensively demonstrated. lncRNA PVT1 (Plasmacytoma Variant Translocation 1) locates on 8q24 region of human chromosome which is known as an oncogenic lncRNA that markedly associated with cancer development and pathophysiology. This investigation aims to explore the expression level of PVT1 in ESCC tissue. Methods: Assessment the expression level of PVT1 in Esophageal squamous cell carcinoma tissues, compared with margin non-tumor tissues (n=40), through the extraction of total cellular RNA, synthesis of cDNA and quantification of the expression of this gene was evaluated by RT-qPCR method. Meanwhile the correlation between this gene expression with clinical parameters in ESCC patient were analyzed. Results: PVT1 was significantly up-regulated in ESCC tissues compared with non-tumor tissues. Up-regulation of PVT1 was positively associated with advanced stages and distance metastasis. (p<0.001) Conclusion: PVT1 was Upregulation in ESCC patients which is associated with advanced Tumor stages (T3 & T4) and distance metastasis. These results may indicate the potential role of PVT1 in ESCC and its possible regulatory role. Thus, further studies are required to identify the possible oncogenic role of this lncRNA and its regulatory mechanism. Exploration of molecular mechanisms behind PVT1 may be useful in designing diagnostic bio-markers and therapeutic interventions.

کلیدواژه ها:

Esophageal Squamous Cell Carcinoma (ESCC) ، lncRNA ، PVT