The effect of curcumine extract on E-cadherin gene expression in adenocarcinoma gastric cancer cells

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 356

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شناسه ملی سند علمی:

CANCERMED04_066

تاریخ نمایه سازی: 17 مرداد 1398

چکیده مقاله:

Introduction &Aim:Cadherin is one of the largest families of membrane proteins. Negative regulation and loss of cadherin function are directly related to the loss of cell adhesion, leading to cancerous cells metastases. Today, the use of herbal medicines is one of the promising ways to treat cancers and prevent the development of cancer. Curcumine has anti-tumor effects and cytotoxic activity against cancer cells and the effect of its extract on the inhibition of many cancers has been observed.Methods:the expression of cadherin in AGS cells treated with curcumine extract was evaluated. AGS cells were cultured in 12 wells plate in DMEM with 10%FBS , 5% Co2 and 85% humidity. Then they were treated with 800, 1200, 2000 μg / ml curcumine extract for 48. RNA extraction, cDNA synthesis was done using kits. Expression of cadherin gene checked using specific primer and real time PCR. Results: The results of real time PCR data have shown that the expression of E-cadherin increased in all concentrations in 48 hours treatment.

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نویسندگان

Ali Habashi

MSc student , Basic Science faculty, Islamic Azad University, Zanjan Branch, Zanjan,Iran.

Zahra Deilami Khiabani

Assistant professor, Basic Science faculty, Islamic Azad University, Zanjan Branch, Zanjan,Iran