Optimization of pectinase activityfrom Piriformospora indicaby sugar beet pulp

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 659

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شناسه ملی سند علمی:

CBC14_005

تاریخ نمایه سازی: 7 اسفند 1396

چکیده مقاله:

Pectinases are the growing enzymes ofbiotechnological sector, showing gradual increase in theirmarket.These enzymes areecofriendly tool of nature that are being used extensively invarious industries likefoodindustry; paperindustry for bleaching of pulp and waste paper recycling; inthe processing of fruit–vegetables, tea–coffee, animal feed;extraction of vegetable oil and scouring of plant fibres.Pectinases as acomplex group of enzymes hydrolyze the pectin-containing substances. They have been used in severalconventional industrial processes; including: juice,textile, plant fiber processing, tea, coffee and oilextraction. Fungal pectinases are among the most important industrial enzymes and are of greatsignificance with widerange of industrial application [1-2]. Sugar-beet pulp (SBP), the main by-productof the sugar refining industry, is especially rich in pectin. In this investigation, weassayed effect ofdifferent temperature (30-90°C) and pH (4-10) on pectinase activity from Piriformosporaindica.First, P.indicawas cultured onKaefer medium supplementedwith SBP powder and maintained at 30 °C underconstant shakingconditions at 200 rpm on an Orbital shaker for 6 days. According to the results theoptimum pH and temperature for pectinase activity was 6 and 40°C, respectively.

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نویسندگان

Parisa Fathi Rezaei

Biology department, Faculty of Science, University of Maragheh, Maragheh, Iran

Somayyeh Kiani

Biology department, Faculty of Science, University of Maragheh, Maragheh, Iran

Saleh Shahabivand

Biology department, Faculty of Science, University of Maragheh, Maragheh, Iran